Bio-orthogonal “Double-Click” Chemistry Based on Multifunctional Coatings

Nanocomposite Based Multifunctional Coatings

Ratiometric analysis of zidovudine (ZDV) incorporation by reverse transcriptases or polymerases via bio-orthogonal click chemistry.

A new fluorescence-based detection method was developed to visualize zidovudine-incorporated DNA using click chemistry. This bio-orthogonal detection method was used to quantify the relative susceptibility of various DNA-synthesizing enzymes toward ZDV incorporation on the basis of ratiometric analysis of band shifts in the gel and the visual observation of cellular DNA.

Bio-Based Coatings for Paper Applications

The barrier resistance and wettability of papers are commonly controlled by the application of petroleum-based derivatives such as polyethylene, waxes and/or fluorderivatives as coating. While surface hydrophobicity is improved by employing these polymers, they have become disfavored due to limitations in fossil-oil resources, poor recyclability, and environmental concerns on generated waste wi...

Imaging Cell Surface Glycosylation in Vivo Using “Double Click” Chemistry

Dynamic alterations in cell surface glycosylation occur in numerous biological processes that involve cell-cell communication and cell migration. We report here imaging of cell surface glycosylation in live mice using double click chemistry. Cell surface glycans were metabolically labeled using peracetylated azido-labeled N-acetylgalactosamine and then reacted, in the first click reaction, with...

Click Chemistry in Peptide-Based Drug Design

Click chemistry is an efficient and chemoselective synthetic method for coupling molecular fragments under mild reaction conditions. Since the advent in 2001 of methods to improve stereochemical conservation, the click chemistry approach has been broadly used to construct diverse chemotypes in both chemical and biological fields. In this review, we discuss the application of click chemistry in ...